GenSolve chemistry recovers high-quality double-stranded DNA from FTA paper and Guthrie cards that can be used for PCR, HLA typing, WGA, genotying, and other downstream applications
GenVault’s scientists have developed a proprietary chemistry called GenSolve to recover DNA from FTA paper and Guthrie cards (aka newborn screening cards). The original sample can be in nearly any form. For example: whole blood, bacterial overnight cultures, glycerol stocks, DMSO stocks, purified genomic DNA, purified plasmid DNA, urine, semen, plant material, and so on. With GenSolve, high-quality, double-stranded DNA up to 35kb in length can be recovered within 1.5 hours.
GenVault scientists pride themselves in having formulated such a robust chemistry for recovering DNA from a cellulosic matrix such as FTA paper and Guthrie cards. Others have taken note of GenSolve as well.
One customer has been using GenSolve to routinely process blood samples collected on FTA cards. This customer’s genetic test, to determine a baby’s gender just a few weeks after conception and well in advance of the first ultrasound, requires the mother to submit blood obtained from a finger prick and spotted on an FTA card. GenSolve is then used by the company’s technicians to recover DNA from the FTA cards. The recovered DNA is of high-quality and yields consistent results in downstream experiments.
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Dr. Janelle Noble of Children’s Hospital Oakland Research Institute (CHORI) recently used GenSolve to recover DNA from blood spotted on newborn screening cards that are nine years old. She was able to recover high-quality, double-stranded DNA from this cards and use the recovered DNA in HLA typing experiments. To read more about this collaboration with Dr. Noble, click here.
