Frequently asked questions about GenTegra™

GenTegra™ is not a filter, beads or paper. GenTegra™ is an inert chemical matrix.

The GenTegra™ matrix is supplied as a transparent coating at the bottom of each GenTegra™ DNA tube. To confirm that the kit you received contains the GenTegra™ matrix, simply rehydrate one tube with 20 µl of hydration solution and take a spectrophotometric reading at a230 to detect the GenTegra matrix.

You may apply more than 20µl of DNA, however, we cannot guarantee that volumes greater than 20µl will dry overnight. For volumes greater than 20µl you will need to determine the optimal drying time for samples in your environment. Ensure that you recover DNA in the same volume that was applied to avoid changing the concentration of the storage solution.

Yes, GenTegra is compatible with samples stored in low-EDTA TE (pH 8 10MM Tris and 0.1MM EDTA), water, Qiagen Buffer AE, TE (pH 7.5, 10MM Tris and 1MM EDTA).

Vortexing is not necessary to solubilize the GenTegra™ matrix and is not recommended. Racks of GenTegra™ DNA tubes may be centrifuged in a plate centrifuge if necessary, but this is not required.

After addition of hydration solution, your samples will be in the same buffer they were stored in at the time of application.

For best results, DNA should be recovered in the same volume that was applied, to avoid changing the concentration of the original storage solution. Recovering DNA in less than 20µl is not recommended.

Store recovered DNA under your typical storage conditions. We recommend storing recovered DNA at 4°C for short-term use and -20°C for long-term storage. Do not store recovered DNA at room temperature.

The GenTegra™ matrix absorbs at 230 nm. Thus, it will not interfere with readings at A260 or A280 and blanking is not required.

Yes, but in order maintain the optimal concentration of GenTegra™ matrix, follow the redrying procedure according to the volume of recovered DNA you removed:

If you removed 1-9µl of recovered DNA, you may redry the remaining DNA in the same GenTegra™ tube. Follow the instructions for drying and storage of DNA on page 9 of the User Manual.

If you removed 10-19 µl of recovered DNA, add an equivalent volume of hydration solution to bring the volume up to 20µl. Apply the sample to a new GenTegra™ tube according to the instructions for DNA application on page 9 of the User Manual.