Application and Analysis Results
Purified DNA stored in GenTegra upon rehydration can be applied directly to the most stringent molecular applications with great ease and simplicity. There is no post-storage purification or sample processing required. One can be assured of confidence in their analysis results by GenTegra’s excellent DNA recovery rate of close to 100%. The GenTegra™ medium is inert and will not interfere in post storage application assays such as PCR, genotyping, and spectrophotometric quantitation. This makes it very easy for GenTegra™ to be integrated in existing equipment, methods, and protocols for analysis, quantitation, and purity determination. Validation studies indicated that GenTegra integrity and recovery is comparative to samples analyzed from cryopreserved samples.
Confidence in Analysis Results
Figure 1. Quality and integrity of DNA stored in GenTegra™ DNA tubes is identical to DNA stored
at -20°C.
Figure 2. Successful QPCR amplification of DNA stored in GenTegra™ DNA tubes. No PCR inhibition was observed in quantitative real-time PCR even when 26% of the reaction volume was made up of DNA (black – DNA stored at -20°C; red, green, yellow – DNA stored in GenTegra™ DNA tubes).
|
control (-20°c) |
GenTegra |
GenTegra (56°c) |
GenTegra |
|
Call Rate |
Affymetrix 6.0 |
99.50% |
99.40% |
99.70% |
99.20% |
Infinium |
99.80% |
99.70% |
99.80% |
99.70% |
|
Concordance |
Affymetrix 6.0 |
99.80% |
97.70% |
99.70% |
|
Infinium |
99.70% |
99.90% |
99.90% |
||
Table 1. Successful genotyping of DNA stored in GenTegra™ DNA tubes via Illumina and Affymetrix genotyping as performed by Expression Analysis, Inc.. Results using Illumina Infinium and Affymetrix 6.0 are identical for DNA stored at -20°C and DNA stored in GenTegra™ DNA tubes.
Illumina Infinium is a registered trademark of Illumina, Incorporated.
Affimetrix Genome-Wide Human SNP Array 6.0 is a product of Affimetrix, Inc.