Recover Biosamples Efficiently using GenSolve

GenSolve

A robust chemistry for recovering high quality double-stranded DNA preserved in Genplates™, FTA Paper and Guthrie cards. GenSolve enables efficient recovery of high quality DNA from biosamples such as blood, buffy coat and plasmid DNA that have been dried on FTA paper and Guthrie cards and banked at ambient temperature. GenSolve is a novel chemistry that ensures excellent recovery of high molecular weight, double-stranded DNA for use with leading downstream molecular analysis platforms. GenSolve utilizes fast and simple protocols that preserve DNA integrity and ensure efficient recoveries.

Gensolve is efficient in recovering DNA from biosamples stored on GenPlates

whole blood
buffy coat
plasmid stocks (stored in glycerol and DMSO)
overnight bacterial cultures

Product Name	Catalog #	Description	Units	List Price
Spin Basket/Tubes	GVSPIN-250			$165.00
Whole Blood DNA Recovery Kit for 4 x 25 reactions	GVR-110			$175.00

Three simple steps to store biosamples and recover DNA

Biosample	Sample Type	Type of GenPlate for Application of Biosample	*GenPlate Catalog #	Type of GenSolve Kit for Recovery	GenSolve Kit Catalog #**
Purified Genomic DNA	Purified DNA	GenPlates for Purified gDNA and plasmid	GVGP-20 GVGNP-20	GenSolve for Purified Genomic DNA, Purified Plasmids, Bacterial Culture, and Glycerol/DMSO Stocks	GVGR-100
Purified Plasmids	Purified DNA	GenPlates for Purified gDNA and plasmid	GVGP-20 GVGNP-20	GenSolve for Purified Genomic DNA, Purified Plasmids, Bacterial Culture, and Glycerol/DMSO Stocks	GVGR-100
Bacterial Culture	Crude Sample (plasmid stock)	GenPlates for Blood and Clones	GVP-20 GVNP-20	GenSolve for Purified Genomic DNA, Purified Plasmids, Bacterial Culture, and Glycerol/DMSO Stocks	GVGR-100
Glycerol Stock	Crude Sample (plasmid stock)	GenPlates for Blood and Clones	GVP-20 GVNP-20	GenSolve for Purified Genomic DNA, Purified Plasmids, Bacterial Culture, and Glycerol/DMSO Stocks	GVGR-100
DMSO Stock	Crude Sample (plasmid stock)	GenPlates for Blood and Clones	GVP-20 GVNP-20	GenSolve for Purified Genomic DNA, Purified Plasmids, Bacterial Culture, and Glycerol/DMSO Stocks	GVGR-100
Whole Blood	Crude Sample	GenPlates for Blood and Clones	GVP-20 GVNP-20	GenSolve for Whole Blood DNA Recovery from FTA paper, Guthrie cards and GenPlate Elements	GVR-110
Blood Products (such as Buffy Coat)	Crude Sample	GenPlates for Blood and Clones	GVP-20 GVNP-20	GenSolve for Whole Blood DNA Recovery from FTA paper, Guthrie cards and GenPlate Elements	GVR-110

*Catalog # for single region GenPlates with or without GenCode
**Catalog # for 100 sample kit(s)

Recover high molecular weight, dsDNA from GenPlates, FTA paper and Guthrie cards spotted with blood products, buccal swabs, mouthwash and Oragene® DNA saliva

Dried Blood Spots
Average Yield*	132 ng
Range	51ng-363ng
Overall CV	11%

* DNA yield is dependent on WBC count of donor sample

Typical yields of dsDNA recovered from 10ul of blood spotted on 6mm disk of FTA paper based on Picogreen dsDNA quantitation analysis.

Recover DNA on genPlates

Figure 1A
The molecular weight of DNA recovered using GenSolve was examined by running 50ng of unamplified DNA directly on a 0.8% agarose gel stained with ethidium bromide. Human genomic DNA from Roche was used as a control.

Figure 1B
Analysis by QPCR confirms reliable amplification of recovered DNA. The reactions amplified a region in exon 3 of the human β-actin gene, which was detected by a FAM-dye-labeled β-actin TaqMan probe. Blue line – 5ng of GenSolve sample; Red line – 5ng of Roche genomic DNA used as a control; Yellow line – No template control

Recover intact plasmids from GenPlates spotted with glycerol stocks, DMSO stocks or bacterial cultures for use in PCR and transformation

GenSolve ensures reliability

Figure 2A
Intact plasmid DNA successfully recovered after storage in GenPlate and recovery using GenSolve. Samples are 20ng BlueScript plasmid with EST insert run on 0.8% agarose gel stained with ethidium bromide.

Figure 2B
pCMV plasmid with EST insert run on 0.8% agarose gel after successful recovery from GenPlate using Gensolve, then amplified with T7 and SP6 primers; positive control is plasmid stock.

Figure 2C
Transformation Efficiency of Plasmid DNA following Storage on GenPlates. 1.0 μg of each purified plasmid (pCMV or pBluescript) was applied to GenPlates and stored for 30 days at room temperature. Plasmid DNA stored at -20°C was used as a control. Plasmids were recovered from GenPlates using GenSolve and used directly for transformation. Equivalent transformation efficiency of 100% was achieved for plasmids stored on GenPlates or at -20°C.

Recover DNA from GenPlates spotted with blood products, buccal swabs, mouthwash or Oragene® DNA/saliva

Recover DNA from GenPlates spotted with glycerol or DMSO bacterial stocks, bacterial cultures, purified genomic DNA or purified plasmid DNA

Click on the links below to download the reference materials:

Citations:

Title:	Assessment of DNA extracted from FTA cards for use on the Illumina iSelect BeadChip
Authors:	McClure MC, McKay SD, Schnabel RD, Taylor JF
Journal:	BMC Res Notes. 2009 Jun 16;2:107
PMID:	19531223
Title:	A general SNP-based molecular barcode for Plasmodium falciparum identification and tracking
Authors:	Daniels R, Volkman SK, Milner DA, Mahesh N, Neafsey DE, Park DJ, Rosen D, Angelino E, Sabeti PC, Wirth DF, Wiegand RC
Journal:	Malar J. 2008 Oct 29;7:223
PMID:	18959790
Title:	Green Technologies for Room Temperature Nucleic Acid Storage
Authors:	Wan E, Akana M, Pons J, Chen J, Musone S, Kwok PY, Liao W
Journal:	Curr Issues Mol Biol. 2009 Oct 2;12(3):135-142
PMID:	19801719
Title:	Establishment of a Sustainable Biobank
Authors:	Arsenault S, McWeeny K, Bilodeau D, Gaudet D
Journal:	GEN. 2008 Jan 15;28(2)